QL-ELE-00-011
|
View in GMO-Matrix | View in GMO-Amplicons |
General information
| Tested in: |
GMO event NK603 | MON-00603-6 (Maize)
|
| Genetic target: | T-nos |
| Description: | Qualitative PCR method for detection of nopaline synthase (nos) terminator (Reiting et al., 2007) |
| Comments: | Element-specific and construct-specific methods may generate amplicons with different sequence or length depending on the GMO events where the element or construct is found. |
Method details
| Specificity: | Element specific |
| Analysis type: | Qualitative |
| Assay: | Real-time PCR (Simplex) |
| Amplicon sequence: |
CATGTAATGCATGACGTTATTTATGAGATGGGTTTTTATGATTAGAGTCCCGCAATTATACATTTAATACGCGATAGAAA ACAA Genbank ID: V00087 |
| Amplicon size: | 84 bp |
| Oligos: |
| Type | Name(s) | Sequence | Length | Target |
|---|---|---|---|---|
| Primer forward | 180-F / | CATGTAATGCATGACGTTATTTATG | 25 bp | T-nos |
| Primer reverse | 180-R / | TTGTTTTCTATCGCGTATTAAATGT | 25 bp | T-nos |
| Probe | Tm-180 / | FAM-ATGGGTTTTTATGATTAGAGTCCCGCAA-TAMRA | 28 bp |