European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record AR232930.1 details
Record ID: AR232930.1
Database: emblrelpat Go to original record
Description: Sequence 1 from patent US 6455759.
Taxon: unidentified
Original size: 4544 bp
Patents: US6455759 (lens, espacenet)

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Method QL-ELE-00-017 details
Method: QL-ELE-00-017 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Barbau-Piednoir et al., 2014).
Target: CaMV P-35S

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Predicted amplification details:

Predicted amplification details
Mismatches Gaps From To Strand Amplicon size
0 0 337 411 + 75 bp

Amplicon size
Amplicon: 75 bp
1     AAAGCAAGTG GATTGATGTG ATATCTCCAC TGACGTAAGG GATGACGCAC
51    AATCCCACTA TCCTTCGCAA GACCC 

View other 5330 matches with an identical amplicon


Primers alignment:

Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 337 359 9.10e-10
primerB 100.0 20 bp 0 0 411 392 4.49e-08


Related publications:

  • Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
  • Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.