JRC GMO-Amplicons | EURL GMFF

Record ID:	MO461056.1
Database:	emblrelpat	Go to original record
Description:	Sequence 808 from patent US 10457954.
Taxon:	unidentified
Original size:	11106 bp
Patents:	US10457954 (lens, espacenet)

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	620	1210	-	591 bp

Amplicon:

591 bp

1     cgaaatcggt agacgctacg gacttgattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcag aaaaaaatta aggtatttct
151   gaaagcgaga ataaaaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaatgaatg gagttgactg cgttggtaga ggactccttc tattcaattt
251   gaatttaaac tccataaaga atgagggata aacctacgat acgtaagtat
301   acgtactgaa atatcaaaga ttctattaat gtcgccctaa aattttttta
351   cataaaaaaa aggaataatt tttgtgaatc tattccaagt tgaaggaaga
401   atcgaatatt cagtaatcaa accattcact ccatagtctg atatatcttt
451   tgacgaactg attaatcgga cgagaataaa gatagagtcc cgttctacat
501   gtcaatatca atacagacaa caatgaaatt tatagtaaga ggaaaatccg
551   tcgactttat aaatcgtgag ggttcaagtc cctctatccc c

View other 5 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.66e-08
primerB	100.0	20 bp	0	0	591	572	2.66e-08

Match details