JRC GMO-Amplicons | EURL GMFF

Record ID:	DQ499400.1
Database:	nt	Go to original record
Description:	Suaeda microsperma tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Suaeda microsperma
Original size:	900 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	558	+	557 bp

Amplicon:

557 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggag
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggtaat cctgagccaa ctcttttttt tcttttgaaa aaagggatag
151   gtgcagagac tcaaaggaag ctgttctaac aaatggggtt ggtttgactg
201   tgttgtttta agaattcttc cgtcaaaact ctatccaaaa acggtgaaga
251   atatactatt gattaatgac accccgaatc tattttgtat ttttttctaa
301   ttttttgata ttttttattt ctatattttt atactcttta atagaaactc
351   tcactaaaca aaaattgaga atatgaaatg aaaatatata tattttctga
401   attggaaaaa agaatcaaat attcattcat caaattcaac actcccgaga
451   ataaagatag agtcccattc tacatgtcaa tactgacaag aatgaaattt
501   atagtaagag gaaaatccgt cgactttcaa aatcagggtt caagtccctc
551   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	557	538	2.51e-08

Match details