Match details
Record ID: |
LT724900.1 |
Database: |
nt |
Go to original record |
Description: |
Gateway expression vector pAGRIKOLA-CATMA2a00256, complete sequence |
Taxon: |
Gateway expression vector pAGRIKOLA-CATMA2a00256 |
Original size: |
10079 bp |
View other 15 matches for this record
Method: |
QL-ELE-00-012 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
Description: | Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim CaMV P-35S) (Waiblinger et al., 2008). |
Target: | CaMV P-35S |
View other 15625 matches for this method
Predicted amplification details: |
Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
1 |
1 |
6454 |
6535 |
+ |
82 bp |
|
1 ctctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51 cgtggaaaaa gaagacgttc caaccacgtc tt
View other 2723 matches with an identical amplicon
Primers and probe alignment: |
Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
primerA |
100.0 |
16 bp |
0 |
0 |
3 |
18 |
5.87e-07 |
primerB |
100.0 |
22 bp |
0 |
0 |
82 |
61 |
3.55e-10 |
probe |
100.0 |
22 bp |
0 |
0 |
21 |
42 |
3.55e-10 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.