JRC GMO-Amplicons | EURL GMFF

Record ID:	OR978440.1
Database:	nt	Go to original record
Description:	Alangium chinense chloroplast, complete genome.
Taxon:	Alangium chinense
Original size:	156673 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48709	49292	+	584 bp

Amplicon:

584 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ccgaaaacaa acaaaggttc
151   agaaagctaa aatcaaaaaa ggataggtgc agagactcaa cggaagctgt
201   tctaacaaat ggagttgact acgttgcgtt attagaagaa tcttttcatc
251   gaaacttcag aaaggatgaa ggataaaact atatacatac gtactgaaat
301   attatatcaa atgattaatg acgacccgaa cctgtatctg tattttttta
351   tatgaaaaat ggaagaattg ttgtgaatcg attccaaatt gaagaaagaa
401   tcaaatattc attcatcaaa tcattcactc catagtctga tagatctttt
451   gaagaactga ttaatcgaac gagaataaag atagagtccc attctacatg
501   tcaataccga caacaatgaa atttatagta agaggaaaat ccgtcgattt
551   tataaatcgt gagggttcaa gtccctctat cccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	584	565	2.63e-08

Match details