JRC GMO-Amplicons | EURL GMFF

Record ID:	PP098463.1
Database:	nt	Go to original record
Description:	Cenchrus purpurascens isolate Liqiu chloroplast, complete genome.
Taxon:	Cenchrus purpurascens
Original size:	138053 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48299	48911	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc cctttttgaa aaaaacaagt ggttctcaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaattac gttgtgttgg taggggaact gcctcgaaat
251   actagaaaga agggctttat acatttaata cacacgtata gatactgaca
301   tagcaaacga ttaatcacgg aacccctatc ataatatggg ttctttattt
351   tctatttttt tctagaatga aattaggaat gattatgaaa tagaaaattc
401   tgaatttttt tagaattatt gtgaatccat tccaatcgaa tattgagtaa
451   tcaaatcctt caattcattg ttttgagatc aaaagtggat taatcgaacg
501   aggataaaga gagagtccca ttctacatgt caatactgac aacaatgaaa
551   tttctagtaa aaggaaaatc cgtcgacttt ataagtcgtg agggttcaag
601   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details