JRC GMO-Amplicons | EURL GMFF

Record ID:	PP159198.1
Database:	nt	Go to original record
Description:	Cenchrus clandestinus chloroplast, complete genome.
Taxon:	Cenchrus clandestinus
Original size:	138001 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48026	48642	+	617 bp

Amplicon:

617 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc cctttttgaa aaaacaagtg gttctcaaac
151   tagaacccaa aggaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacgaatcga agtaattacg ttgtgttggt aggggaactc cctcgaaata
251   ctagaaagaa gggctttata catttaatac acacgtatag atactgacat
301   agcaaacgat taatcacaga acccctatca tatcataata tgggttcttt
351   attttctatt tttttctaga atgaaattag gaatgattat gaaatagaaa
401   attctgaatt tttttagaat tattgtgaat ccattccaat cgaatattga
451   gtaatcaaat ccttcaattc attgttttga gatcaaaagt ggattaatcg
501   aacgaggata aagagagagt cccattctac atgtcaatac tgacaacaat
551   gaaatttcta gtaaaaggaa aatccgtcga ctttataagt cgtgagggtt
601   caagtccctc tatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	617	598	2.78e-08

Match details