JRC GMO-Amplicons | EURL GMFF

Record ID:	PP159194.1
Database:	nt	Go to original record
Description:	Cenchrus longisetus chloroplast, complete genome.
Taxon:	Cenchrus longisetus
Original size:	138554 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48181	48789	+	609 bp

Amplicon:

609 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc cctttttgaa aaacaagtgg ttctcaaact
151   agaacccaaa ggaaaaggat aggtgcagag actcaatgga agatgttcta
201   acgaatcgaa gtaattacgt tgtgttggta agtgaactcc ctcgaaatac
251   tagaaagagg ggctttatac atttaataca cacgtataga tactgacata
301   gcaaacgatt aatcacggaa ccccttaata taggttcttt attttatatt
351   tttttttcta gaatgaaatt aggaatgatt atgaaataga aaattctgaa
401   tttttttaga attattgtga atccattcca atcgaatatt gagtaatcaa
451   atccttcaat tcattgtttt gagatcaaaa gtggattaat cgaacgagga
501   taaagagaga gtcccattct acatgtcaat actgacaaca atgaaatttc
551   tagtaaaagg aaaatccgtc gactttataa gtcgtgaggg ttcaagtccc
601   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	609	590	2.74e-08

Match details