JRC GMO-Amplicons | EURL GMFF

Record ID:	PP159191.1
Database:	nt	Go to original record
Description:	Cenchrus massaicus chloroplast, complete genome.
Taxon:	Cenchrus massaicus
Original size:	137929 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48223	48834	+	612 bp

Amplicon:

612 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc cctttttgaa aaaacaagtg gttctcaaac
151   tagaacccaa aggaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacgaatcga agtaattacg ttgtgttggt aggggaactg cctcgaaata
251   ctagaaagaa gggctttata catttaatac acacgtatag atactgacat
301   agcaaacgat taatcacgga acccctatca taatatgggt tctttatttt
351   atattttttt ctagaatgaa attaggaatg attatgaaat agaaaattct
401   gaattttttt agaattattg tgaatccatt ccaatcgaat attgagtaat
451   caaatccttc aattcattgt tttgagatca aaagtggatt aatcgaacga
501   ggataaagag agagtcccat tctacatgtc aatactgaca acaatgaaat
551   ttctagtaaa aggaaaatcc gtcgacttta taagtcgtga gggttcaagt
601   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	612	593	2.76e-08

Match details