JRC GMO-Amplicons | EURL GMFF

Record ID:	PP159189.1
Database:	nt	Go to original record
Description:	Cenchrus longissimus chloroplast, complete genome.
Taxon:	Cenchrus longissimus
Original size:	138134 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48030	48643	+	614 bp

Amplicon:

614 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tgataacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaaacaagt ggttctaaaa
151   ctagaaccca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg aagtaattac gttgtgttgg taggggaact ccctcgaaat
251   actagaaaga agggctttat acatttaata cacacgtata gatactgaca
301   tagcaaacga ttaatcacag aacccctatc ataatatagg ttctttattt
351   tatatttttt tctagaatga aattaggaat gattatgaaa tagaaaattc
401   tgaatttttt ttagaattat tgtgaatcca ttccaatcga atattgagta
451   atcaaatcct tcaattcatt gttttgagat caaaagtgga ttaatcgaac
501   gaggataaag agagagtccc attctacatg tcaatactga caacaatgaa
551   atttctagta aaaggaaaat ccgtcgactt tataagtcgt gagggttcaa
601   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	614	595	2.77e-08

Match details