JRC GMO-Amplicons | EURL GMFF

Record ID:	PP153940.1
Database:	nt	Go to original record
Description:	Periploca calophylla chloroplast, complete genome.
Taxon:	Periploca calophylla
Original size:	153717 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47252	47817	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtaaggaa
51    acctactaag tgatgacttt caaattcaga gaaaccccgg aattaagaaa
101   aagggcaatc ctgagccaaa tcctatttta cacaaacaaa ggttcagaaa
151   acgaaaacaa ggataggtgc agagactcga cggaagatgt tctaacaaat
201   ggaggtggcc acgttcgtag agaaatcctt ccatcgaaaa ttcagaaagg
251   atgaaggata aacacatata catacgtatt gaatactata tcaaatgatt
301   atgccgaccc gaatgaatcc gtattttttc tataaaaaac ggaagaattg
351   gtgtgagtcg attctttctt caatgtggaa tcgaatattc attgatcaaa
401   tgattcactc catagtctgt agatcccctt ttcaagaacg gattaatcgg
451   acgagaataa aggtagagtc ccgttctaca tgtcaatgct ggcaacaatg
501   aaatttatag taagaggaaa atccgtcgac ttaaaaaatc gtgagggttc
551   aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details