JRC GMO-Amplicons | EURL GMFF

Record ID:	KX078545.1
Database:	nt	Go to original record
Description:	Rhodiola rosea genotype Mengusovska dolina tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Rhodiola rosea
Original size:	921 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	585	+	585 bp

Amplicon:

585 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tggtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ttcttttccg aaaccaaacc caaatacagg
151   ggttcaaaaa gcgcgaataa aaaaggatag gtgcagagac tcaacggaag
201   ttgttctaac aaatagagtt tactatgttg cattgacaaa ggaatctttt
251   cattgaaact ccagaaagga tgaaggataa atcttaataa acatatgtat
301   acctaccgaa atagtatatc aaatgattaa tgacgactca aatttttatt
351   ttaaaatgaa agaattgtta ggaagcgatt ccgagttgaa gaaagaatcg
401   actcttcatt gataaaataa gtgtcactcc acagtctgag agatcttttg
451   acgaactgag attaatcgga cgagaataaa gatagagtcc cattatacat
501   gtcaatactg acaacaagga aattgatagt aaaaggaaaa tccgtcgact
551   ttagaaatca tgagggttca agtccctcta tcccc

View other 29 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.63e-08
primerB	100.0	20 bp	0	0	585	566	2.63e-08

Match details