JRC GMO-Amplicons | EURL GMFF

Record ID:	KT597274.1
Database:	nt	Go to original record
Description:	Tetrastigma cf. ellipticum PBP-2017 isolate 3249 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Tetrastigma cf. ellipticum PBP-2017
Original size:	969 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	579	+	578 bp

Amplicon:

578 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct taatatggaa
51    acctactaag tgataacttt caaattcagg gaaaccctgg aataaaaaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccggaa aacgctaata
151   aaaaagaaag gataggtgca gagactcaat ggaagctgtt ctaacaaaaa
201   caaatggagt tgactatgtt acttaaatga atccttccat caaaacttca
251   gaaccagaaa ggatgaagga taaacctata tacataggta ctggtaatga
301   aatattatat caaatgatta atgatggctc gaatctctat ttttttatat
351   gaaaaatgaa agaattgttg taaatcgatt ccaaaaaaaa agaatcgaat
401   attcattgat caaataattc actccatagt ccgatagatc atttgaagaa
451   cggattaatc ggacgagaat aaagagagag tcccattcta catgtcaata
501   tcgacagcaa tgaaatttat agtaagagga aaatccgtcg actttaaaaa
551   tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.60e-08
primerB	100.0	20 bp	0	0	578	559	2.60e-08

Match details