JRC GMO-Amplicons | EURL GMFF

Record ID:	KR259146.1
Database:	nt	Go to original record
Description:	Amorimia pellegrinii voucher 612(HUEFS) tRNA-Leu gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe gene, partial sequence; chloroplast.
Taxon:	Amorimia pellegrinii
Original size:	1111 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	507	1111	-	605 bp

Amplicon:

605 bp

1     cgaaatcggt agacgctacg gatttaattg gaatgagcct tggtatggaa
51    acttantaag tgataacytt tcaannttca gagaaaccct ggaattaaaa
101   awgggcaatc ctgagccaaa tcctgttttc aaaaaacaaa caaaggttct
151   taaagacaga aataaaggga taggtgcaga gactcaacgg aagctgttct
201   aacaaawgga gttgactgcc tttcattagg aaaggaatct tttcgttaaa
251   atatcagatt cagataaggg atagccccat atacatatta tatgtactaa
301   aatagtctat caaatgacta atgaaatgac gacccgaatc tctatttatt
351   tcattttttt ttgaatagaa aaaaggttgt tttgaatcka ttccaagttg
401   aagaaaagat tgaatattaa ttcttgatca aaacattcac tccatagtct
451   gatagataac cgaagtctga tagataaccg attaatcaga cgagaataaw
501   gatagagtcc cctttcacat gtcaatatcg acawcaagga aatttagagt
551   aataggaaaw tccgtcgact ttaaaaatcg ggagggttca agtccctcta
601   tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	605	586	2.73e-08

Match details