JRC GMO-Amplicons | EURL GMFF

Record ID:	KR259142.1
Database:	nt	Go to original record
Description:	Amorimia septentrionalis voucher 1161(UNIVASF) tRNA-Leu gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe gene, partial sequence; chloroplast.
Taxon:	Amorimia septentrionalis
Original size:	1094 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	497	1093	-	597 bp

Amplicon:

597 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcaa aaaacaaaca aaggttcata
151   aagacagaaa taaagggata ggtgcagaga ctcaacggaa gctgttctaa
201   caaatggagt tgactgcctt tcattaggaa aggaatcttt tcgttaaaat
251   atcagattca gataagggat agccccatat acatattata tgtactaaaa
301   tagtctatca aatgactaat gaaatgacga cccgaatctc tatcattttt
351   ttttgaatag aaaaaaggtt gttttgaatc gattccaagt tgaagaaaag
401   attgaatatt aattcttgat caaaacattc actccatagt ctgatagata
451   accgaagtct gatagataac cgattaatca gacgagaata aagatagagt
501   cccctttcac atgtcaatat cgacaacaag gaaatttaga gtaataggaa
551   aatccgtcga ctttagaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	597	578	2.69e-08

Match details