JRC GMO-Amplicons | EURL GMFF

Record ID:	KR259141.1
Database:	nt	Go to original record
Description:	Amorimia concinna voucher 9292(NY) tRNA-Leu gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe gene, partial sequence; chloroplast.
Taxon:	Amorimia concinna
Original size:	985 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	386	982	-	597 bp

Amplicon:

597 bp

1     cgaaatcggt agacgctacg gacttaattg gatgagcctt ggtatggaaa
51    cttattaagt gataactttc aaattcagag aaaccctgga attaaaaatg
101   ggcaatcctg agccaaatcc tgttttcaaa aaacaaacaa aggttcataa
151   agacagaaat aaagggatag gtgcagagac tcaacggaag ctgttctaac
201   aaatggagtt gactgccttt cattaggaaa ggaatctttt cgttaaaata
251   tcagattcag ataagggata gccccatata catattatat gtactaaaat
301   agtttatcaa atgactaatg aaatgacgac ccgaatctct atcatttttt
351   tttgaataga aaaaaggttg ttttgaatwg attccaagtt gaagaaaaga
401   ttgaatatta attyttgatc aaaacattca ctccatagty tgatagwtaa
451   cmgaagtytg atagataacc gwttaatcag tcgagaatwa agatagantc
501   ccctttcaca tgtcaatwtm nacaacaagg aaatttagag tnaataggaa
551   aatccstcga ctttagaaat cgtgakggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	597	578	2.69e-08

Match details