JRC GMO-Amplicons | EURL GMFF

Record ID:	PP112995.1
Database:	nt	Go to original record
Description:	Meconopsis torquata chloroplast, complete genome.
Taxon:	Meconopsis torquata
Original size:	153290 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46696	47294	+	599 bp

Amplicon:

599 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcag aaaacaaatt aagggatttc
151   tgaaagcgag aataaaaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaatgaat ggagttgact gcgttggtag aggaatcctt ctattaaatt
251   tgaattgaaa ctccataaag aatgagggat gaacctatat acgatacgta
301   cgtatacgta ctgaaatatc aaagattcta ttaatgccgc cctaaatata
351   tattttttta cataaaaaaa atataatttt tgtgaatcta ttccaagttg
401   aaggaagaat tgaaaattca gtgatcaaac cattcactcc atagtctgat
451   agatcttttg acgaactgat taatcggacg agaataaaga tagagtccca
501   ttctacatgt caatatcaat accgacaaca atgaaattta tagtaagagg
551   aaaatccgtc gactttagaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.70e-08
primerB	100.0	20 bp	0	0	599	580	2.70e-08

Match details