JRC GMO-Amplicons | EURL GMFF

Record ID:	PP102709.1
Database:	nt	Go to original record
Description:	Penstemon digitalis chloroplast, complete genome.
Taxon:	Penstemon digitalis
Original size:	152993 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47185	47756	+	572 bp

Amplicon:

572 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgataacttt caaattcaga gaaaccccgg aatgaataaa
101   aatgggcaat cctgagccaa atcctatgct tctcaaaata aaggttcaaa
151   aagcgaaaat aagggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg actgcattgg tagatgaatc ttttcatcga aacttcagaa
251   aggatgaagg ataaacgtat ctattgaata ctagaatcaa attcttaatg
301   atgccccgaa tctgtatctg tatttttcta tatgaaaaat ggaagaattg
351   gtgtcaattg attccacatt gaaaaaagaa tcgaatattc attcatcaaa
401   tcattcactc catagtctga tagttctttt aaagaactga ttaatcggac
451   aagaataaag atagagtccc attctacatg tcaatatgtc aataccggca
501   acaatgaaat ttatagtaag aggaaaatcc gtcgacttta aaaatcgtga
551   gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	572	553	2.58e-08

Match details