JRC GMO-Amplicons | EURL GMFF

Record ID:	PP069005.1
Database:	nt	Go to original record
Description:	Melampyrum laxum chloroplast, complete genome.
Taxon:	Melampyrum laxum
Original size:	143906 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	71443	71991	-	549 bp

Amplicon:

549 bp

1     cgaaatcggt agacgctacg gacttaatta aattgagcct tggtatggca
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aatgggcaat cctgagccaa ctcctgtttt ctcaaaacaa agattcagaa
151   aacaaaaaaa ggataggtgc agagactcta tggaagttgt tctaacaaat
201   ggagttgatt acgctggtag aggaatgaaa cttccgaaag gataaaggca
251   tctattgaat actatatcaa tatcaataaa ttgattaatg atggcccaaa
301   tctgtatctg tatttttgaa tacgcaaaag ggaagaagta gtgtgaattg
351   attccatatt gaaaaaagaa gattcattga tcaaataatt cactccataa
401   tccgatagat cgtttaaaaa actgattaat cggacgagaa taaagataga
451   gtcccattct acatgtcaat accggcaaca atgaaattta tagtaagagg
501   aaaatccgtc gacttttaaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	549	530	2.47e-08

Match details