JRC GMO-Amplicons | EURL GMFF

Record ID:	PP069004.1
Database:	nt	Go to original record
Description:	Lathraea japonica chloroplast, complete genome.
Taxon:	Lathraea japonica
Original size:	143840 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	98682	99229	-	548 bp

Amplicon:

548 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaagaaa
101   aatgggcaat cctgagccaa atcctgtttt cttaaaacaa agattcagaa
151   aacgaaaaaa aaggataggt gcagagactc tatggaagtt gttctaacaa
201   atggagttga ttacgctggt agaggaatga aacttcagaa aggataaagg
251   atcagggcat ctattgaata ctatatcaat aagttgatta atgatggccc
301   aaatctatat cttttgaata cacaaaatgg aagaagcggt gtgacttgat
351   tccatattga aaaaagaaga ttcattgatc aaataattca ctccatagtc
401   cgattagatc gtttaaagaa ctgattaatc ggacgagaat aaagatagag
451   tcccgttcta catgtcaata ccggcaacaa tgaaatttat agtaagagga
501   aaatccgtcg actttaaaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	548	529	2.47e-08

Match details