JRC GMO-Amplicons | EURL GMFF

Record ID:	OR832244.2
Database:	nt	Go to original record
Description:	Physostegia virginiana chloroplast, complete genome.
Taxon:	Physostegia virginiana
Original size:	152880 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49145	49712	+	568 bp

Amplicon:

568 bp

1     cgaaatcggt agacgctacg gacttaatta gaattcaatt gagccttggt
51    gtgggaaacc tactaagtga taactttcaa attcagagaa accccggaat
101   taataaaaat gggcgatcct gatccaaatc ctgttttttc aaaacaaagg
151   ttcaaaaaat gaaataacga aataaaggat aggtgcagag actcaatgga
201   agctgttcta acaaatggag ttgactgcgt tggtagagga attctttcta
251   cggaaacttc agaaaggatg aagaataaac gtatctatcg aatactatat
301   caaatgatta atgatggccc gatttgaata tgaaaaacag aagaattggt
351   gtgaattgat tctacattga aattgaagaa aaaatagaat attcattcat
401   caaatcattc actccatagt ccgatagatc ttttaaagaa ctgattaatc
451   ggacgagaat aaagatagag tcccattcta catgtcaata ccggcaacaa
501   tgaaatttat agtaagagga aaatccgtcg actttaaaaa tcgtgagggt
551   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	568	549	2.56e-08

Match details