JRC GMO-Amplicons | EURL GMFF

Record ID:	ON641272.1
Database:	nt	Go to original record
Description:	Mentha pulegium isolate BPTPS116 voucher LISE:96437 chloroplast, complete genome.
Taxon:	Mentha pulegium
Original size:	152281 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47054	47619	+	566 bp

Amplicon:

566 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt cccaaaacaa aggttgcaaa
151   aaacgaaaaa aaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgtcggt agaggaatct ttccatggaa aattttataa
251   aggatgaaaa ataaacgcat ctattgaata ctatatcaaa tgattaatgt
301   tggcccgaat ctattttttt aatatgaaaa tgaaaaaatg gaaaaatcgg
351   tgtgaatttt tttcacgttg aagaaaaaat agaatattca tcaactcatt
401   cactccgtag tccgatagat cttttaaaga acttattaat cggacgagaa
451   taaagataga gtcccattct acatgctaca tatcaatacc ggcaacaatg
501   aaatttatag taagaggaaa atccgtcgac tttaaaaatc gtgagggttc
551   aagtccctct atcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	566	547	2.55e-08

Match details