JRC GMO-Amplicons | EURL GMFF

Record ID:	ON641269.1
Database:	nt	Go to original record
Description:	Thymbra capitata isolate BPTPS101 voucher LISE:96423 chloroplast, complete genome.
Taxon:	Thymbra capitata
Original size:	151475 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46340	46907	+	568 bp

Amplicon:

568 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggtttcaaa
151   aaacgaaaaa aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgactgcgtc ggtagaggaa tctttccatg gaaactttat
251   aaaggatgaa agataaacgc atctattgaa tactatatca aatgattaat
301   gttggcccga atctattttt ttaatatgaa aatgaaaaaa tggaaaaatc
351   ggtgtgaatt tatttcacgt tgaagaaaaa atagaatatt catcaactca
401   ttaactccat agtccgatag atcttttaaa gaacttatta atcggacgag
451   aataaagata gagtcccatt ctacatgcta catatcaata ccggcaacaa
501   tgaaatttat agtaagagga aaatccgtcg actttaaaaa tcgtgagggt
551   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	568	549	2.56e-08

Match details