JRC GMO-Amplicons | EURL GMFF

Record ID:	PP093763.1
Database:	nt	Go to original record
Description:	Leonotis leonurus chloroplast, complete genome.
Taxon:	Leonotis leonurus
Original size:	151587 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46573	47133	+	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttaatta taattaaatt gagccttagt
51    gtgggaaacc tactaagtga taactttcaa attcagagaa accccggaat
101   taataaaaat gggcgatcct gatccaaatc ctagtttttc aaatcaaaac
151   aaaagttcaa aaaacgaaat aaaaggatag gtgcagagac tcaatggaag
201   ttgttctaac aaatggagtt gactacgttg gtagaggaat cctttctacg
251   gaaacttcag aaaggataac cgtatctatc gaatactata tcaaatgatt
301   aatgatggcc cgaatccgta tttttaatat gaaaaataga agaattggtg
351   tgaattgatt ctatattgaa gaaaaaatag actattcatt catcaaatca
401   ttcactccat agtccgatag atcttttaaa gaactgatta atcggacgag
451   aataaagata gagtcccatt ctacatgtca ataccggcaa caatgaaatt
501   tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc
551   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details