JRC GMO-Amplicons | EURL GMFF

Record ID:	PP072314.1
Database:	nt	Go to original record
Description:	Paspalum vaginatum chloroplast, complete genome.
Taxon:	Paspalum vaginatum
Original size:	140483 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48910	49526	+	617 bp

Amplicon:

617 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc ccttttttga aaaacaagtg gttctcaaac
151   tagaacccaa aagaaaagga taggtgcaga gactcaatgg aagctgttct
201   aacgaatcga agtaattgcg ttgtgttggt agtggaacct cttcgaaatt
251   agagaaagaa gggctttata catctaatat acacgcatat atactgacat
301   agcaaacgat taatcacgga acccatatca taatataggt tccttatttt
351   attttttgaa tgaaattagg aatgattatg aaatagaaaa ttctgcattt
401   ttttagaatt attgtgaatc cattccaatc gaatattgag taatcaaatc
451   cttcaattca ttattttgtt ttcgagatct taaaaaaagt ggattaatcg
501   gacgaggata aagagagagt cccattctac atgtcaatac tgacaacaat
551   gaaatttcta gtaaaaggaa aatccgtcga ctttataagt cgtgagggtt
601   caagtccctc tatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	617	598	2.78e-08

Match details