JRC GMO-Amplicons | EURL GMFF

Record ID:	PP078786.1
Database:	nt	Go to original record
Description:	Gardenia jasminoides f. longicarpa isolate Z2 chloroplast, complete genome.
Taxon:	Gardenia jasminoides f. longicarpa
Original size:	154921 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47888	48437	+	550 bp

Amplicon:

550 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat tctgagccaa atcccatttt ccgaaaccaa aggtgaaaaa
151   aggataggtg cagagactca acggaagctg ttctaacaaa tggagttggc
201   tgcattagta gagaaatctt tccatcgaaa attccgaaag tataaagtga
251   aggataaacg tatatatacg tattgaatac tatatcaaat gattaatgac
301   gactcgactg aatctgtatt ttttatatga aaacggaaga gaatagattc
351   cacattgaag aaagaatcga atattcattg atcaaatgat tcactccata
401   gtctgataga tcttttcaag aattgattaa tcggacgaga ataaagatag
451   agtcccgttc tacatgtcaa tgtcggcaac aatgaaattt atagtaagag
501   gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc ctctatcccc

View other 17 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	550	531	2.47e-08

Match details