JRC GMO-Amplicons | EURL GMFF

Record ID:	PP073955.1
Database:	nt	Go to original record
Description:	Ajuga reptans chloroplast, complete genome.
Taxon:	Ajuga reptans
Original size:	149825 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47123	47677	+	555 bp

Amplicon:

555 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctgctaag tgataacttt caaattcaga gaaacccagg aactaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa agattcaaaa
151   aacgaaaaaa gggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttcac tgcgctggta gaggaatctt tccatggaaa cttcagaaag
251   gatgaaggat aaatggatct attgaatact atatcgaatg attcacgatg
301   gcccgaatct gtatttttta atatgaaaaa tggaagaatt ggtgtgaatt
351   gattccacat tgaagaaaaa atcgaatatt cattcatcaa atcattcact
401   ccatagtccg atagatcttt tcaagaactg attaatcgga cgagaataaa
451   gatagagtcc cgttctacat gtcaataccg gcaacaatga aatttatagt
501   aataggaaaa tccgtcgact ttaaaaatcg tgagggttca agtccctcta
551   tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	555	536	2.50e-08

Match details