JRC GMO-Amplicons | EURL GMFF

Record ID:	MN756858.1
Database:	nt	Go to original record
Description:	Hylomecon japonica var. dissecta voucher cp07 chloroplast, complete genome.
Taxon:	Hylomecon japonica var. dissecta
Original size:	159942 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49919	50515	+	597 bp

Amplicon:

597 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaat
101   gggcaatcct gagccaaatc ctgttttcag aaaacaaaaa aggtttatga
151   aagcgagaat aaaataaaaa agggataggt gcagagactc aatggaagct
201   gttctaatga atggagttga ttgcgttgcg ttggtagagg aatctttcta
251   ttgaaactcc agaaagaata aaggatgaac ctatatagga tacatacgta
301   tacgtactga aatatcaaac gattaatgac gccccaaatc tttatttttt
351   tatatgaaaa atggaataat tcttgtgaat caattccatt ccaagttgaa
401   ggaagaatcg aatattcagt gatcaaatca ttcgctccat agtctgatag
451   atcttttgcc gaactgatta atcagacgag aataaagata gagtcccgtt
501   ctacatgtca atatcaatac cgacaacaat gaaatttata gtaagaggaa
551   aatccgtcga ctttaaaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	597	578	2.69e-08

Match details