JRC GMO-Amplicons | EURL GMFF

Record ID:	OR992089.1
Database:	nt	Go to original record
Description:	Oreocharis qingyunshanensis chloroplast, complete genome.
Taxon:	Oreocharis qingyunshanensis
Original size:	153485 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47988	48542	+	555 bp

Amplicon:

555 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaagacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa gggttcagag
151   agcgaaaaag aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac cgcgttggta gaagaatcga aacttcagaa aggatgaagg
251   ataaacgtat ctattgaata ctattgatga ttaatgatgg cccgaatctc
301   tatctgggtt tttgtatatg aaaaatagaa gaattggtgt gaattgattt
351   cacattgata aaagaatcga atattcatta atcaaatcat tcactccaca
401   gtccgataga tctcttaaag aacttattat tttattcgga cgagaataaa
451   gatagagtcc cgttctacat gtcaataccg gcaacaatga aacttatagt
501   aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca agtccctcta
551   tcccc

View other 3 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	555	536	2.50e-08

Match details