JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ818609.1
Database:	nt	Go to original record
Description:	Elephantopus scaber isolate XSK20220055 chloroplast, complete genome.
Taxon:	Elephantopus scaber
Original size:	151909 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46764	47289	+	526 bp

Amplicon:

526 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcacgtttt ccgaaaacaa acaaaggttc
151   agaaagcgaa aagaaaaaag aagaaaaaag gataggtgca gagactcgat
201   ggaagctgtt ctaacgagtg gagttgattg tcttacgtta ttagaggaat
251   ccttctatcg aaacttcaga aaagatgaag gataaacctg tatacataat
301   accgaagatt tgttgtgaat cgattccata ttgaagaaag aatcgaatat
351   tcattgatca aaccattcac tccagaatct gatagatctt ttgaagaact
401   gattaatcgg acgagaataa agatagagtc ccgttctaca tgtcaatccc
451   ggcaacaatg aaatttatag taagaggaaa atccgtcgat ttcaaaaatc
501   gtgagggttc aagtccctct atcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.37e-08
primerB	100.0	20 bp	0	0	526	507	2.37e-08

Match details