JRC GMO-Amplicons | EURL GMFF

Record ID:	MT559304.1
Database:	nt	Go to original record
Description:	Amaranthus tuberculatus voucher GZW chloroplast, complete genome.
Taxon:	Amaranthus tuberculatus
Original size:	150680 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47412	48025	+	614 bp

Amplicon:

614 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggag
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggtaat cctgagccaa ctcctttttc aaaagtaaaa aaaaatacgg
151   attcagaaag caagaatcaa aaaaaggata ggtgcagaga ctcaaaggaa
201   gctgttctaa caaatggagt tgattgtgct gggttgttcg aagaattctt
251   ccgtcgaaat tcctgattaa tgacacttcg aatctattct gtatttctat
301   ttttagaatt tttagatatt tcttatttct agataatata gaatatgaaa
351   ttttctagaa ataaaaatga atatgaaaaa atggaagaat ttttgtgact
401   ctatttcaag ttgaaaaaag aatcaaatat tcatcaaatc attcactcca
451   tagtctgata gatctttctt tgaaagaacc ggttaatcgg ttactcggac
501   gagaataaag atagagtccc attatacatg tcaatactga caacaatgaa
551   atttatagta cgaggaaaat ccgtcgactt taaaaatcgt gagggttcaa
601   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	614	595	2.77e-08

Match details