JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ267655.1
Database:	nt	Go to original record
Description:	Petrocodon sp. LF-2023a isolate WF160 trnL-UAA gene, trnL-trnF intergenic spacer, and trnF-GAA gene, complete sequence; chloroplast.
Taxon:	Petrocodon sp. LF-2023a
Original size:	969 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	560	+	550 bp

Amplicon:

550 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaagacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa gggttcagag
151   agcgaaaaag aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac cgcgttggta gaaaaattga aacttcagaa aggatgaagg
251   ataaacgtat ctattgaata ctattgatga ttaataatgg cccgaatctg
301   tatctgggtt tttgtatatg aaaaatagaa gaattggtgt gaattgattt
351   cacattgata aaagaatcga atatttatta atcaaataat tcactccaca
401   gtccgataga tctcttaaag aacttattat tcggacgaga ataaagatag
451   agtcccgttc tacatgtcaa taccggcaac aatgaaactt atagtaagag
501   gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	550	531	2.47e-08

Match details