JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ348024.1
Database:	nt	Go to original record
Description:	Kalopanax septemlobus plastid, complete genome.
Taxon:	Kalopanax septemlobus
Original size:	156502 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49735	50320	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ccgaaaacaa acaaaggttc
151   agaaggcgaa aaagggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtg gactgtgttg cattggtaga ggaatccttc cattgaaact
251   tccaaaagga tgaaagataa acgtatatac atacgtatac gtactgaaat
301   actctatcaa atgattaatg acgatccgaa tctgtatttt ttatatgaaa
351   acggaagaat tgttgtgaat cgattccata ttgacgaaag aatcgaatat
401   cgaatattca ttgatcaaat cattcacccc atacaaagtc tgatagttct
451   tttgaagaac tgattaattg gacgagaata aagatagagt cccattctac
501   atgtcaatac cggcaacaat gaaatttata gtaagaggaa aatccgtcga
551   ctttaaaatc gtgagggttc aagtccctct atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details