JRC GMO-Amplicons | EURL GMFF

Record ID:	ON121986.1
Database:	nt	Go to original record
Description:	Verbascum phoeniceum chloroplast, complete genome.
Taxon:	Verbascum phoeniceum
Original size:	153273 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47934	48506	+	573 bp

Amplicon:

573 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   ggcgaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttgact gcgttggtag aggaatcttt ccatcgaaac ttcagaaagg
251   gtgacggata aacgtatcta ttgaatacta tatcaaatga ttaatgatgg
301   cccgaatctg tatctttttt ttttttttta tatatatgaa aaatggaaaa
351   attgatgtga atttattcca cattgaagaa agaatcaaat aaaatattca
401   ttcatcaaat cattcactcc atagtctgat agatctttta aagaactgat
451   taatcggacg agaataaaga tagagtccca ttctacatgt caatatcggc
501   aacaatgaaa tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg
551   agggttcaag tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	573	554	2.58e-08

Match details