JRC GMO-Amplicons | EURL GMFF

Record ID:	ON121985.1
Database:	nt	Go to original record
Description:	Verbascum chaixii chloroplast, complete genome.
Taxon:	Verbascum chaixii
Original size:	153495 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48003	48567	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctatttt ctcaaaacaa aggttcagaa
151   ggcgaaaaaa ggataggtgc agagactcaa tggaagctgt tctaacaaat
201   ggagttgact gcgttggtag aggaatcttt ccatcgaaac ttcagaaagg
251   gtgacggata aacgtatcta ttgaatacta tatcaaatga ttaatgatgg
301   cccgaatctg tatctttttt tttttatatg aaaaatggaa aaatcgatgt
351   gaatttattc cacattgaag aaagaatcaa ataaaatatt cattcatcaa
401   atcattcact ccatagtctg atagatcttt taaagaactg attaatcgga
451   cgagaataaa gatagagtcc cattctacat gtcaatatcg gcaacaatga
501   aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details