JRC GMO-Amplicons | EURL GMFF

Record ID:	ON950076.1
Database:	nt	Go to original record
Description:	Clinopodium barosmum chloroplast, complete genome.
Taxon:	Clinopodium barosmum
Original size:	152261 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	47032	47598	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacca aaggtttcaa
151   aaaacgaaaa aaagggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gactgcgtcg gtagaggaat ctttccatgg aaactttata
251   aaggatgaaa gataaacgca tctattgaat actatatcaa atgattaatg
301   ttggcccgaa tctatttttt taatatgaaa atgaaaaaat ggaaaaatcg
351   gtgtgaattt ttttcacgtt gaagaaaaaa tagaatattc atcaactcat
401   tcactccata gtccgataga tcttttaaag aacttattaa tcggacgaga
451   ataaagatag agtcccattc tacatgctac atatcaatac cggcaacaat
501   gaaatttata gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt
551   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details