JRC GMO-Amplicons | EURL GMFF

Record ID:	HG515539.2
Database:	nt	Go to original record
Description:	Orobanche californica chloroplast complete genome, specimen voucher PAC:S.Wicke Oc54
Taxon:	Aphyllon californicum
Original size:	123739 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	31262	31818	+	557 bp

Amplicon:

557 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagccc tggtatgaaa
51    acctactaag tggtaacttt caaattcaga gaaaccccgg aattaataaa
101   aaagggcaat cctgagccaa atcctttttt atcaaaacaa agcttcagaa
151   agcgcaaata aaggataggt gcagagactc aatggaagct attctaaaaa
201   atgtagttga tagcgttggt agaggaatct ttccctcaga acttcagaaa
251   gtatgaagga taaacgtatc tattgaatac tatatcaaat gattaatgat
301   ggctcgaacc tttctctgta ttttttaata ttaaaggagg aagaattgat
351   gtgaattgat tccatattga agaaagaata tttattcatc aaatcattca
401   ctccacagtc cgatagatct tttaaagatc tgattaatcg aaagagaata
451   aagatagagt cccattctac atgtcaatgc cggcaacaat taaagttata
501   gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt caagtccctc
551   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	557	538	2.51e-08

Match details