JRC GMO-Amplicons | EURL GMFF

Record ID:	KP326399.1
Database:	nt	Go to original record
Description:	Salvia limbata voucher li.36 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Salvia limbata
Original size:	903 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	559	+	559 bp

Amplicon:

559 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggcgtt gactgcgccg gtagaggatt ccatggaaac tttagttaga
251   aaggatgaag gataaacaca tctattgaat actatattaa atgattaatg
301   ttggcccgaa tctgtttttt taatatgaaa atgaaaaaat aggtgtgaat
351   ttatttcacg ttgaagaata aatcgaatat tcctcaattc attcactcca
401   tagtccgata gatcttttaa agaacttatt aatcggacga gaataaagat
451   agagtcccat tctacatgct acatgtcaat accggcaaca atgaaattta
501   tggtaagagg aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc
551   tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	559	540	2.52e-08

Match details