JRC GMO-Amplicons | EURL GMFF

Record ID:	KP326395.1
Database:	nt	Go to original record
Description:	Salvia virgata voucher vi.20 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Salvia virgata
Original size:	908 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	569	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gactgcgccg gtagaggatt ccatggaaac tttagaaagg
251   atgaaggata aacacatcta ttgaatacta tattcaaatt aaatgattaa
301   tgttgacccg aatctgtttt tttaatatga aaatgaaaaa atggaaaaat
351   aggtgtgaat ttatttcacg ttgaagaata aatcgaatat tcctcaattc
401   attcactcca tagtccgata gatcttttaa agaacttata aatcggacga
451   gaataaagat agagtcccat tctacatgct acatgtcaat accggcaaca
501   atgaaattta tggtaagagg aaaatccgtc gactttaaaa atcgtgaggg
551   ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details