JRC GMO-Amplicons | EURL GMFF

Record ID:	KP326393.1
Database:	nt	Go to original record
Description:	Salvia sharifii voucher sh.3 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Salvia sharifii
Original size:	896 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	552	+	552 bp

Amplicon:

552 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aggataggtg cagagactca atggaagctg ttctaacaaa
201   tggagttgac tgcgccggta gaggattcca tggaaacttt agaaaggatg
251   aaggataaac acatctattg aatactatat taaatgatta atgttgaccc
301   gaatctgttt ttttaatatg aaaatgaaaa aataggtgtg aatttatttc
351   acgttgaaga ataaatcgaa tattcctcaa ttcattcact ccatagtccg
401   atagatcttt taaagaactt attaatcgga cgagaataaa gatagagtcc
451   cattctacat gctacgtgtc aataccggca acaatgaaat ttatggtaag
501   aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt ccctctatcc
551   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	552	533	2.48e-08

Match details