JRC GMO-Amplicons | EURL GMFF

Record ID:	KP326390.1
Database:	nt	Go to original record
Description:	Salvia nemorosa voucher ne.17 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Salvia nemorosa
Original size:	915 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	567	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccag aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgccggt agaggattcc atggaaactt tagaaaggat
251   gaaggataaa cgcatctatt gaatactata tttaaattaa atgattaatg
301   ttgacccgga tctttttttt taatatgaaa atgaaaaaat ggaaaaatag
351   gtgtgaattt atttcacgtt gaagaataaa tcgaatattc ctcaactcat
401   tcactccata gtccgataga tcttttaaag aacttattaa tcggacgaga
451   ataaagatag agtcccattc tacatgctac atgtcaatac cggcaacaat
501   gaaatttatg gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt
551   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details