JRC GMO-Amplicons | EURL GMFF

Record ID:	KP326388.1
Database:	nt	Go to original record
Description:	Salvia ceratophylla voucher ce.14 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Salvia ceratophylla
Original size:	912 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	568	+	568 bp

Amplicon:

568 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg actgcgccgg tagaggattc catggaaact ttagaaagga
251   tgaaggataa tcacatctat cgaatactat attaaaatta aatgattaat
301   gttgacccga atctgttttt ttaatatgaa aatggaaaaa tggaaaaata
351   ggtgtgaatt tatttcacgt tgaagaataa atcgaatatt cctcaattca
401   ttcactccat agtccgatag atcttttaaa gaacttatta atcggacgag
451   aataaagata gagtcccatt ctacatgcta catgtcaata ccggcaacaa
501   tgaaatttat ggtaagagga aaatccgtcg actttaaaaa tcgtgagggt
551   tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	568	549	2.56e-08

Match details