JRC GMO-Amplicons | EURL GMFF

Record ID:	KP326387.1
Database:	nt	Go to original record
Description:	Salvia hydrangea voucher hy.26 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Salvia hydrangea
Original size:	904 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	560	+	560 bp

Amplicon:

560 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aacgaaaaaa aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgactgcgcc ggtagaggaa tctttccatg gaaactttag
251   aaaggatgaa ggatacacgc atctattgaa tactatatca aatgattaat
301   gttgacccga atctgttttt ttaatatgaa aatggaaaaa taggtgtgaa
351   tttatttcac gttgaagaat aaatcgaata ttcctcaact cattcactcc
401   atagtccgat agatctttta aagaacttat taatcggacg agaataaaga
451   tagagtccca ttctacatgc tacatgtcaa taccggcaac aatgaaattt
501   atggtaagag gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc
551   ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.52e-08
primerB	100.0	20 bp	0	0	560	541	2.52e-08

Match details