JRC GMO-Amplicons | EURL GMFF

Record ID:	KP326385.1
Database:	nt	Go to original record
Description:	Salvia multicaulis voucher mu.44 tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Salvia multicaulis
Original size:	897 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	554	+	554 bp

Amplicon:

554 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcaaaa
151   aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa caaatgagtt
201   gactgcgccg gtagagaaat ctttccatgg aaactttaga ttagaaagga
251   tgaaggataa acgcatctat tgaatactat atcaaatgat taatgttgac
301   ccgaatctgt ttttttaata tgaaaatgga aaaataggtg tgaatttatt
351   tcacgttgaa gaataaatcg aatattcctc aactcattca ctccatagtc
401   cgatagatct tttaaagaac ttattaatcg gacgagaata aagatagagt
451   cccattctac atgctacatg tcaataccgg caacaatgaa atttatggta
501   agaggaaaat ccgtcgactt taaaaatcgt gagggttcaa gtccctctat
551   cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.49e-08
primerB	100.0	20 bp	0	0	554	535	2.49e-08

Match details