JRC GMO-Amplicons | EURL GMFF

Record ID:	KX752090.1
Database:	nt	Go to original record
Description:	Alloteropsis angusta isolate Asem1 chloroplast, complete genome.
Taxon:	Alloteropsis angusta
Original size:	140206 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48657	49269	+	613 bp

Amplicon:

613 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtacggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaaa
101   gggcaatcct gagccaaatc ccttttttga aaaaataagt ggttgtaaaa
151   ctaaaaccca aaggaaaagg ataggtgcag agactcgatg gaagctgttc
201   taacgaatcg aagtaattac gttgtgttgg tagtggaact ccctggaaat
251   tagagaaaga agggctttat acatctaata cacacgtata gatactgaca
301   tagcaaacga ttaatcacgg aacccatatc ataatatatg ttctttattt
351   tattttttag aatgaaatta ggaatgatta tgaaatagaa aattcagaat
401   ttttttagaa ttattgtgaa tccattccaa ttgaatattg agtaatcaaa
451   tccttcaatt cattgttttg agatctttaa aaaagtggat taatcgaacg
501   aggataaaga gagagtccca ttctacatgt caatactgac aacaatgaaa
551   tttctagtaa aaggaaaatc cgtcgacttt ataagtcgtg agggttcaag
601   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.76e-08
primerB	100.0	20 bp	0	0	613	594	2.76e-08

Match details