JRC GMO-Amplicons | EURL GMFF

Record ID:	OR866441.1
Database:	nt	Go to original record
Description:	Knorringia sibirica chloroplast, complete genome.
Taxon:	Knorringia sibirica
Original size:	161384 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	49617	50237	+	621 bp

Amplicon:

621 bp

1     cgaaatcggt agacgctacg gacttaattg gtttgagcct tagtatggaa
51    acctactaag tgagaacttt caaattcaga gaaaccctgg aataaaaaaa
101   aatgggcaat cctgagccaa ctccttcttt tcaaaaggaa aaataaaaaa
151   aggataggtg cagagactca atggaagctg ttctaacaaa tggagttgac
201   ggtcttgcgt tgttagaaga attcgcccat cgaaactcca aaaaggatga
251   agaataaacc tagacgcata cgtacttaaa cttaaatcta aataatacta
301   tatttatatt ctaaaatata aatattctat aaaatagaat attaaaatta
351   aatgaaaaat attgatgacg acccgaatct ttattttatg actatgaaaa
401   agtggaagaa ttgttgtgaa tcgattccaa gttgaagaaa gaatcgaata
451   ttcgtttagt aaattaaagc atttattccc cacttttgaa gacccgatcc
501   accggatgag aatgaagata gagtcccatt ctacgtgtca atcctgacaa
551   caatgaaatt tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag
601   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.80e-08
primerB	100.0	20 bp	0	0	621	602	2.80e-08

Match details