JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ672941.1
Database:	nt	Go to original record
Description:	Phlomoides eriocalyx plastid, complete genome.
Taxon:	Phlomoides eriocalyx
Original size:	150667 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46028	46589	+	562 bp

Amplicon:

562 bp

1     cgaaatcggt agacgctacg gacttaatta taattaaatt gagccttagt
51    gtgggaaacc cactaagtga taactttcaa attcagagaa accccggaat
101   taataaaaat gggcgatcct gatccaaatc ctgttttttc aaaacaaagg
151   ttcaaaaaac aaactaaagg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttgactgc gttggtagag gaatcctttc tacggaaact
251   tcagaaagga tgaaggataa acgtatctat cgaatactat atcaaatgat
301   taatgatggc ccgaatccct atttttaata tgaaaaatag aagaattggt
351   gtgaattgat tctatattga agaaaaaatc gaatattcat tcatcaaatc
401   attcactcca tagtccgata gatcttttaa agaactgatt aatcggacga
451   gaataaagat agagtcccat cctacatgtc aatactggca acaatgaaat
501   ttatagtaag aggaaaatcc gtcgatttta aaaatcgtga gggttcaagt
551   ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	562	543	2.53e-08

Match details