JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ672936.1
Database:	nt	Go to original record
Description:	Phlomoides pedunculata plastid, complete genome.
Taxon:	Phlomoides pedunculata
Original size:	152010 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46830	47384	+	555 bp

Amplicon:

555 bp

1     cgaaatcggt agacgctacg gacttaatta taattaaatt gagccttagt
51    gtgggaaacc tactaagtga taactttcaa attcagagaa accccggaat
101   taataaaaat gggcgatcct gatccaaatc ctgttttttc aaaacaaagg
151   ttcaaaaaac gaactaaagg ataggtgcag agactcaatg gaagctgttc
201   taacaaatgg agttgactgc gttggtagag gaatcctttc tacggaaact
251   tcagaaagga taaacgtatc tatcgaatac tatataaaat gattaatgat
301   ggcccgaatc cgtattttta atatgaaaaa tagaaaaatt ggtgtgaatt
351   tattctatat tgaagaaaaa atcgaatatt cattcatcaa atcattcact
401   ccatagtccg atagatcttt taaagaactg attaatcgga cgagaataaa
451   gatagagtcc cattctacat gtcaatactg gcaacaatga aatttatagt
501   aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca agtccctcta
551   tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.50e-08
primerB	100.0	20 bp	0	0	555	536	2.50e-08

Match details