JRC GMO-Amplicons | EURL GMFF

Record ID:	OQ791227.1
Database:	nt	Go to original record
Description:	Neomicrocalamus sp. CYZ-2023a chloroplast, complete genome.
Taxon:	Neomicrocalamus sp. CYZ-2023a
Original size:	139214 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	48752	49365	+	614 bp

Amplicon:

614 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagt ggttctcgaa
151   ctagaatcca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg agttgattac gttgtgttgg tagtggaact ccctctaaat
251   tagagaaagt aaggggttta tacatctaat acacacatat ggatactgac
301   atagcaaacg attaatcaca gaacccatat cataatatag gttctttatt
351   cttttttaga atgaaattag gaatgattat gaaatagaaa attcagaatt
401   ttttttagaa ttattgtgaa tccatcccaa tcgaatattg agtaatcaaa
451   tccttcaatt catagttttc gagatctttt aaaaagtgga ttaatcggac
501   gaggataaag agagagtccc attctacatg tcaatactga caacaatgaa
551   atttctagta aaaggaaaat ccgtcgactt tataagtcgt gagggttcaa
601   gtccctctat cccc

View other 5 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	614	595	2.77e-08

Match details